Evaluation of the effectiveness and safety of TransPRK assisted by smart pulse technology for high myopia / 中华实验眼科杂志

Objective:To evaluate the effectiveness and safety of transepithelial photorefractive keratectomy (TransPRK) assisted by smart pluse technology (SPT) for the correction of high myopia.Methods:An observational case series study was conducted.Sixty high myopic patients (107 eyes) with spherical equivalent (SE)≥-6.0 D who received TransPRK assisted by SPT from January to December 2016 in Eye Hospital of Wenzhou Medical University were enrolled.Uncorrected visual acuity (UCVA) and best corrected visual acuity (BCVA) of the patients were examined and recorded in logarithm of the minimum angle of resolution (LogMAR) units, and refraction was examined with a subjective refractometer.The healing of corneal epithelium and corneal haze was observed with a slit lamp.Intraocular pressure (IOP) was measured with the non-contact tonometer.Safety index (SI) and efficacy index (EI) were analyzed.The follow-up time was 12 months.This study protocol adhered to the Declaration of Helsinki and was approved by an Ethics Committee of Eye Hospital of Wenzhou Medical University (No.2019-197-k-177). Written informed consent was obtained from each patient prior to any medical examination.Results:The mean epithelial healing time was (3.77±1.02) days.There were statistically significant differences in UCVA and BCVA between before and after surgery ( Z=380.812, 267.313; both at P<0.001). And the 7-day, 6-month, and 12-month postoperative BCVA were better than preoperative BCVA, showing statistically significant differences (all at P<0.05). Mean SI was 1.10±0.12, and mean EI was 1.05±0.17 at 12 months after surgery.There was no significant difference between the attempted SE before surgery (-8.02±1.36)D and the achieved SE at 12 months after surgery (-8.04±1.51)D ( P=0.523). SE in the predictive range within ±0.50 D accounted for 79% (85/107) and that within ±1.0 D accounted for 92% (98/107). The IOP was slightly increased in 3 eyes at 7 days and 7 eyes at 1 month after surgery, respectively, which returned to normal after the use of ophthalmic solution for lowing IOP.The incidence of haze severer than grade 1 was less than 1% (1 eye), and haze gradually disappeared after application of drugs. Conclusions:TransPRK assisted by SPT for high myopia shows good safety, effectiveness and predictability.It is an ideal corneal surface surgery to correct high myopia.

Application of Chinese VF-11R questionnaire in presenting low vision cataract patients undergoing surgery / 中华实验眼科杂志

Objective:To assess the applying value of Chinese shorter version of the Visual Function Index questionnaire (VF-11R-CN) for presenting low-vision cataract pre-operation and post-operation by using minimal clinically important difference (MCID) determined by combination of distribution-based with anchor-based methods, and to compare the improvement rate of vision-related quality of life (VRQol).Methods:A cross-sectional study was performed in this study.Ninety-eight patients with presenting low vision cataract were enrolled at Eye Hospital of Wenzhou Medical University from May 2018 to June 2019.All the patients received a phacoemulsification with intraocular lens implantation.A questionnaire survey was carried out with VF-11R-CN Scale before surgery and 1 month-3 months after surgery, and a complement anchor item was added during the questionnaire after surgery.The applicability of the scale was assessed by floor or ceiling effects, and score difference of the VF-11R-CN over time and effective size was determined.Distribution-based MCID was calculated using 0.5 standard deviation (SD) of score difference and 1.96 standard error of measurement (SEM). Anchor-based MCID was calculated using the slope of the linear regression analysis.In responder analysis, sensitivity and specificity of MCID was reported.The differences of postoperative VRQol improvement rate based-on MCID were compared between male and female, single eye disease and multiple eye diseases, pre-operative and post-operative scores.This study protocol was approved by the Ethics Committee of Eye Hospital of Wenzhou Medical University, and followed the Declaration of Helsinki.Written informed consent was obtained from each patient prior to any medical examination.Results:A total of 85 patients completed the following up.The mean score difference was 399.51±234.92, and that of the presenting visual acuity (PVA) was 0.65±0.36.Both the minor floor and ceiling effects were 1.18% before surgery, and the ceiling effects were 22.35% after surgery.The score before surgery was significantly higher than that after surgery ( t=15.68, P<0.001). The effective size for the surgery was 1.63.The MCID was 122.23 and 123.10 according to 0.5 SD and 1.96 SEM.The linearity regression analysis showed that score difference reduced 106.17 if anchor item option increased 1.The average MCID estimate was 117.17.A total of 76 patients (89.41%) reported an improvement of VRQol.The sensitivity of MCID for the assessment of VRQol was 96.67% and specificity was 46.67%.No significant differences were found in VRQol improvement rate between gender, single cataract and multiple eye diseases or PVA (LogMAR 1.3 as cutoff value) ( P=0.73, 0.88, 0.27). Conclusions:VF-11R-CN scale is available for Chinese presenting low vision cataract patients undergoing surgery.The MCID value for the cataract surgery is 117.17 scores, with a high sensitivity and a relatively low specificity.

Efficacy and safety of pazufloxacin mesylate eye drops for bacterial conjunctivitis:a multicenter randomized, double-masked, parallel controlled clinical trial / 中华实验眼科杂志

Objective To compare the clinical efficacy and safety following the topical application of pazufloxacin mesylate eye drops with levofloxacin eye drops (LOFX) for bacterial conjunctivitis.Methods A multicenter,randomized,investigator-masked,parallel-controlled clinical trial was performed.Five hundred and twenty eyes of 520 patients with bacterial conjunctivitis were enrolled from March to October 2008 in seven ophthalmic centers in China.The patients were randomized into trial group and control group in 3 ∶ 1 ratio with the 390 eyes in the trial group and 130 eyes in the control group.Pazufloxacin mesylate eye drops was topically used 4 times per day for consecutively 7-14 days in the trial group,and levofloxacin eye drops was used in the same way in the control group.Microbiological cultures for conjunctiva sac secretions and drug sensitive test were carried out before and at the end of the administration of eye drops.The signs and symptoms were observed and scored before treatment and 0,3,7 and 14days after treatment.The adverse events following the administration of the eye drops were evaluated and compared.Results The intention to treat analysis (ITT) showed that the cure rate was 59.38％ in the trial group and 60.47％ in the control group,with the effective rate 88.80％ and 86.05％,respectively,with an insignificant difference between the two groups (x2 =0.12,P =0.72).The clinically evaluable analysis (CE) exhibited that the cure rates were 63.48％ in the trial group and 63.87％ in the control group,with the effective rate 92.46％ and 88.24％,whichwas not significantly different between them (x2 =0.54,P=0.46).The modified-ITT analysis (mITT) showed that the cure rates were 60.57％ in the trial group and 62.07％ in the control group,with the effective rate 90.32％ and 88.51％,without significant difference between the two groups (P＞0.05).Based on microbiologically evaluable analysis (ME),the clinical cure rates were 63.71％ and 63.41％ in the trial group and control group,and the effective rates were 93.44％ and 90.24％,respectively.There was no significantly difference between the both groups (P＞0.05).In the trial group and control group,the bacterial eradication rate was 89.42％ and 90.80％ based on ITT,90.11％ and 92.77％ based on CE,respectively.There was no significant difference in incidences of adverse events following the administration of the drug between the trial group and control group,including ocular tolerance,burning sensation,pricking and itching (P =0.34).Conclusions The effectiveness and adverse response were resemble between Pazufloxacin mesylate eye drops and LOFV following the topical application for bacterial conjunctivitis,which indicate that Pazufloxacin mesylate eye drops is effective and safe.

Comparison of visual quality following different methods of YAG laser posterior capsulotomy for posterior capsular opacification / 中华实验眼科杂志

Objective To evaluate and compare the subjective and objective visual quality of YAG laser posterior capsulotomy.Methods A series of case observation study was designed.Twenty-eight patients (38 eyes)underwent Nd:YAG laser posterior capsulotomy in the Eye Hospital of Wenzhou Medical University were retrospectively collected from 2015 to 2016.Patients were divided into circular capsulotomy group (20 patients 23 eyes) and cruciate capsulotomy group (12 patients 15 eyes) based on the capsulotomy technique (round incision of posterior capsule or cross incision of posterior capsule).The uncorrected visual acuity (UCVA) and best corrected visual acuity (BCVA) were examined before and 2 weeks after operation,the intraocular pressure was also measured before operation,1 hour after operation and 2 weeks after operation.The Strehl ratio (SR),modulation transfer function (MTF) cut-off,objective scatter index (OSI),OQAS values (OVs) were analyzed by Two-channel visual quality analysis system OQAS Ⅱ at 1 h and 2 weeks after operation.The changes of intraocular scattering and visual quality at 1 h and 2 weeks after operation with different posterior capsulotomy methods were compared and analyzed.Results At 2 weeks after operation,the UCVA was 0.18±0.15 and 0.16±0.14,and the BCVA was 0.10±0.10 and 0.10±0.11,respectively in the circular capsulotomy group and cruciate capsulotomy group,which was higher than those before operation (all at P＜0.05),but there was no significant difference in UCVA and BCVA between the two groups (all at P＞0.05).There was no significant difference in intraocular pressure between the two groups at 1 hour and 2 weeks after operation (both at P＞0.05).In the circular capsulotomy group,the incidence of black shadow drift was 39.1％ and 30.4％ respectively at 1 hour and 2 weeks after operation,which was higher than that in the cruciate capsulotomy group (6.7％ and 6.7 ％),with no significant differences between the two groups (P =0.065,0.177).No significant difference were found in the incidence of black shadow drift before eyes between the two groups (P＞0.05),and there was no significant differences in the OQAS parameters OSI,MTF,SR,OV100％,OV20％ and OV9％ between the two groups (all at P＞0.05).Conclusions After Nd:YAG laser capsulotomy,the incidence of black shadow drift in the cruciate capsulotomy group is lower than that in the circular capsulotomy group.There is no significant difference in the visual acuity,intraocular pressure,intraocular scattering and visual quality parameters between circular posterior capsulotomy and cross posterior capsulotomy.

Correlation of visual field with corneal changes in angle closure glaucoma / 中华全科医师杂志

Forty four patients (84 eyes)with primary chronic angle closure glaucoma,including 14 cases(26 eyes)with mild visual field defect,22 cases(42 eyes)with moderate defect and 8 cases(16 eyes) with severe defect,treated in our hospital from January 2014 to January 2016 were enrolled in the study.The visual field defect (MD),corneal endothelial cell density (ECD) and central corneal thickness (CCT) were measured.The average CCT in mild,moderate and severe visual field defect groups was (531.4 ± 27.1) μm,(521.2 ± 31.4) μm and (513.3 ± 28.2) μm,respectively (F =-3.24,P =0.04).The mean endothelial cell density in three groups was (2 635.0 ± 320.3) cells/mm2,(2 619.2 ± 161.4) cells/mm2 and(2 589.7 ± 290.4) cells/mm2,respectively (F =0.22,P =0.80).The results indicate that with the aggravation of visual field defect in patients with chronic angle closure glaucoma,the central corneal thickness is significantly thinner,and the mean density of central corneal endothelial cells shows a downward trend.Close observation and active intervention are needed for chronic angle closure glaucoma with thin central corneal thickness.

Clinical results of transepithelial corneal collagen cross-linking for pediatric keratoconus / 中华实验眼科杂志

Background Keratoconus is a progressive corneal thinning and protrusion disease that develop in the age of puberty,resulting in a certain extent impairment of visual function.Corneal collagen cross-linking (CXL) increases the stiffness of the cornea through the photooxidation of ultraviolet A (UVA) and riboflavin,with the aim to postpone and prevent the progression of keratoectasia.Objective This study was to evaluate the safety and efficacy of transepithelial CXL in the treatment of pediatric keratoconus.Methods Ten eyes of 9 pediatric patients with keratoconus undergoing transepithelial CXL were enrolled from February 2010 to March 2013 in Affiliated Eye Hospital of Wenzhou Medical University,with the mean age was (15± 1) years (range from 13 years to 17 years).After topical anesthesia (0.1％ tetracaine) for 15 minutes,0.5％ riboflavin was applied until it was saturated in the anterior chamber,then UVA with the intensity of 3 mW/cm2 was performed on the cornea for 30 minutes.The uncorrected distance visual acuity (UDVA),corrected distance visual acuity (CDVA),refractive error,topography,corneal thinnest thickness were measured 7 days,1 month,3 months,6 months and 1 year after operation.Endothelium cell density (ECD) was measured 7 days afier operative.Results The mean corneal reepithelization time was (1.4±0.8) days.The UDVA and CDVA were significantly improved from preoperative 1.02±0.16 and 0.34±0.20 to postoperative 0.77±0.18 and 0.25 ±0.15,respectively (t =4.251,3.750;both at P＜0.05).The refractive sphere and spherical equivalent significantly changed from preoperative (-7.15±3.00)D and (-9.26±3.23)D to postoperative (-5.28±2.05) D and (-7.05±2.08) D 1 year,respectively (t =-2.515,-2.597;both at P＜0.05).Maximum Kvalue (Kmax) was significantly decreased from preoperative (64.1 ± 11.9) D to postoperative (61.8 ± 10.4) D (t =2.304,P＜0.05).The refractive cylinder,corneal thinnest thickness and ECD showed no significant differences between preoperation and postoperation (t =-1.331,0.328,1.205;all at P＞0.05).Stromal opacity was observed in 2 eyes 3 months and 6 months after operation,respectively.Conclusions Transepithelial CXL is safe and effective in prolonging or halting progression in adolescent keratoconus.

Effect of stiffness of polyelectrolyte multilayer on titanium surface on bacterium adhesion / 中华口腔医学杂志

<p><b>OBJECTIVE</b>To provide a theoretical basis for surface modification of titanium implants, the effects of the stiffness of polyelectrolyte multilayer films on titanium surface on bacterium adhesion was explored.</p><p><b>METHODS</b>Via layer-by-layer technique, catechol functionalized polyelectrolyte multilayer film (cPEM) was constructed on titanium surface by using catechol functionalized hyaluronic acid (cHA) and lipopolysaccharide-amine nanopolymersomes (NP). The stiffness of cPEM was controlled by adjusting the catechol substitution degree of cHA (5%, 10%, 20%, 30%, 40%, 50%, 60%, 70%). Titanium samples covered with cPEM were selected as test group, and the cPEM was constructed with the lowest, medium and highest stiffness. The polished titanium was used as a control. The surface topography of titanium before and after film construction was observed by scanning electron microscopy (SEM). At 1 and 24 h after incubation, the adhesion and clonal formation of Streptococcus mutans (S. mutans) on different titanium surfaces were quantified, and their morphology and survival status were observed by SEM and laser scanning confocal microscope (LSCM).</p><p><b>RESULTS</b>When the catechol grafting ratio was 5%, 30% and 70%, the lowest, medium and highest cPEM stiffness were obtained, and the cPEM stiffness were (10.69±4.54) GPa(cPEM-L), (20.99± 5.81) GPa (cPEM-M) and (32.57±6.93) GPa (cPEM-H) respectively, and the stiffness of polished titanium was (107.12±8.68) GPa (P<0.05). SEM observation showed that after cPEM coating, the titanium surface became smoother. After incubation for 1 and 24 h, the amount of adhesion and clonal formation of S. mutans on cPEM were higher than those on control titanium, and the difference was statistically significant (P<0.05). SEM images showed that for 1 h incubation, softer surfaces were beneficial for S. mutans adhering and agglomerating, while this difference nearly disappeared at 24 h. Observation under LSCM revealed that most of bacteria were alive on titanium disks at 1 h, and their amount decreased with the increase of stiffness. At 24 h, the living/dead bacterium ratios on cPEM-L and control titanium was higher than that on cPEM-M and cPEM-H, and cPEM-L surface was dominated by living bacteria, while stiffer cPEM-M and cPEM-H had more dead bacteria than living bacteria.</p><p><b>CONCLUSIONS</b>Increasing the stiffness of polyelectrolyte films on titanium limits the adhesion of S. mutans. As an independent factor, stiffness influences the bacterium adhesion.</p>

Repeatability of corneal power measurements by Galilei Ⅱ dual Scheimpflug analyzer in post-LASIK eyes / 中华实验眼科杂志

Background The assessment of total corneal power is essential to both the prediction of intraocular lens power before cataract surgery and the diagnosis of corneal ectasia.Objective This study was to access the repeatability of corneal power measurements in post-laser assisted in-situ keratomileusis (LASIK) eyes with GalileiⅡ dual Scheimpflug analyzer.Methods In this cross-sectional study,68 eyes of 36 subjects who received LASIK surgery more than 3 months in Eye Hospital of Wenzhou Medical University were recruited from July to September in 2011.The mean preoperative spherical equivalent diopter was (-4.95±2.08) D in the subjects with the diopter range from-1.75 D to-10.00 D.The parameters of corneal power were measured by Galilei Ⅱ dual Scheimpflug analyzer,including SimK,Ka,Kp,KG and Kray.Three-time consecutive measurements were completed in every examined eye to obtain the data of SimK,Ka,Kp,KG and Kray,respectively.The coefficient of variation (CV),standard deviation (SD),Cronbach α coefficient and intra-class correlation coefficient (ICC) were calculated to evaluate the repeatability.Concordance between the different measurements was assessed using Bland-Altman agreement analysis.This study followed the Helsinki Declaration,all subjects signed informed consent.Results The CVs of three-time measured SimK,Ka,Kp,KG and Kray were 0.24％,0.28％,-0.37％,0.31％ and 0.32％,respectively,and the Cronbach α values were ＞0.9 and the ICCs were ＞0.982.The Bland-Altman analysis between different measurements showed that the maximum 95％ limits of agreement (LoA) were 0.37 D for SimK,0.46 D for Ka,0.09 D for Kp,0.45 D for KG and 0.49 D for Kray,respectively.Conclusions The Galilei Ⅱ dual Scheimpflug analyzer performed high repeatability and concordance on corneal power measurements in post-LASIK eyes.The differences between measuring results are clinically acceptable.

Research Progress of Molecular Imprinting Cryogel / 分析化学

Cryogel is a kind of elastic, spongy hydrogel prepared via addition polymerization from monomers or cross-linking reaction from a polymer at a temperature lower than the freezing point of the solvent. Cryogel has been principally used in molecular imprinting cryogel based solid phase extraction in the field of analytical chemistry, which combines both the advantages of high selectivity of the molecular imprinting and the porous structure, high throughput of a cryogel. Molecular imprinting cryogel can be used as the stationary phase in a packed column, a monolith column, or the adsorbent in a separation membrane. A variety of molecules can act as template molecules, including small molecules, macromolecules, a certain fragment of a macromolecule, and even an inorganic ion. Both a simple template and a complex template can be used. When employing more than a molecule ( or an ion) as templates simultaneously, the method is termed as Mixed template. While high abundance components of a complex sample act directly as mixed template, we name the method as Pending template. Further, while an analogue, an isotopic label, or a fragment of the target substance acts as a template, the method is defined as Dummy template. However, the research on cryogel is insufficient and the application of the cryogel is narrow hitherto.

Study on construction and biological effects of lipopolysaccharide-amine nanopolymersomes/hyaluronic acid polyelectrolyte multilayer films on titanium surface / 中华口腔医学杂志

<p><b>OBJECTIVE</b>To provide a basis for surface modification of polyelectrolyte multilayer films (PEM) on implants by exploring the effects of immobilization of PEM on titanium surfaces on their cell biological effects.</p><p><b>METHODS</b>By using plasmid of bone morphogenetic protein-2 (pBMP-2)-loaded lipopolysaccharide-amine nanopolymersomes (pLNP) as cationic polyelectrolytes and hyaluronic acid (HA) as anionic polyelectrolytes. PEM were constructed on alkaline-heat treated titanium surfaces via layer by layer self-assembly(LbL) technique. A successive deposition of HA and pLNP on titanium surfaces was defined as one cycle of assembly. The morphology of titanium surface before and after assembly treatment was observed by scanning electron microscopy (SEM). The ultraviolet (UV) spectra and surface contact angles of PEM with different self-assembly cycles were measured. The adhesion and proliferation of mesenchymal stem cell (BMSC) on surfaces of group A (4 cycles of assembly, with outermost layer of pLNP), group B (4.5 cycles of assembly, with outermost layer of HA), blank control (polished titanium, Ti group) and alkaline-heat treated titanium (Ti-OH group) were investigated. Cell differentiation indexed by alkaline phosphatase activity (ALP) and in situ transfection of BMSC on surfaces of group A, Ti, negative control [4 cycles of assembly without pBMP- 2] were evaluated.</p><p><b>RESULTS</b>Self assembly of PEM made the titanium surface become relatively smooth. DNA absorption peaked appear at 260 nm in UV spectra, and the absorption intensity increased with assembly, suggesting the successful construction of PEM. After alkali- heat treatment, the surface contact angle of titanium decreased from (62.6±4.9) ° to (8.1±2.2) °. During LbL, with alternately introducing pLNP and HA, the contact angle increased in a jagged mode at a gradually decreased rate, which further proved the success of assembly. Cell adhesion for group A at 0.5 and 1 h was 0.415±0.085 and 0.426±0.048, which was significantly higher than those for group B (0.299±0.012, 0.355±0.022), Ti-OH group (0.225±0.007, 0.260±0.010) and Ti group (0.302±0.056, 0.339±0.028) (P < 0.01). Cell proliferation for group A and B at 3, 5 and 7 d were significantly higher than those for Ti and Ti-OH group (P < 0.01). ALP in group A at day 7 was 261±58, which was significantly higher than those in group B and Ti group. Group A had in situ transfection capability for BMSC.</p><p><b>CONCLUSIONS</b>PEM with good cell biological effects can be constructed successfully on titanium surfaces using gene- loaded lipopolysaccharide- amine nanopolymersomes and hyaluronic acid.</p>

Angiogenic activity of alginate-graft-PEI/pVEGF complexes in vivo / 生物工程学报

To study the angiogenic activity of amphoteric brush-type copolymer complex of alginate-graft-PEI/pVEGF (Alg-g-PEI/pVEGF) in vivo, we evaluated the toxicity of Alg-g-PEI/pVEGF complexes to rMSCs and zebra fish first. Then, we used gel retardation assay to investigate the protection of complex to pDNA against DNase I, serum and heparin. For in vivo study, we evaluated the angiogenic activity of Alg-g-PEI/pVEGF complexes by using CAM and zebra fish as animal models, PEI 25K/pVEGF and saline as positive and negative controls. Our results show that Alg-g-PEI protected pVEGF from enzymolysis and displacement of heparin in some degree, and its complexes with pVEGF were less toxic to rMSCs and zebra fish. Alg-g-PEI/pVEGF complexes induced significant angiogenesis, which was dosage-dependent. In CAM, when the dosage of pVEGF was 2.4 microg/CAM, Alg-g-PEI group achieved the maximum of angiogenesis, and the area ratio of vessel to the total surface was 44.04%, which is higher than PEI 25K group (35.90%) and saline group (24.03%) (**P < 0.01). In zebra fish, the angiogenesis increased with the increase of N/P ratios of Alg-g-PEI/pVEGF complexes in our studied range; when N/P ratio was 110, the optimal angiogenesis was obtained with vessel length of 1.11 mm and area of 1.70 x 10(3) pixels, which is higher than saline group (0.69 mm and 0.94 x 10(3) pixels) (**P < 0.01) and PEI 25k group (0.82 mm and 1.11 x 10(3) pixels) (**P < 0.01). Our results demonstratethat Alg-g-PEI/pVEGF significantly induces angiogenesis in CAM and zebra fish, and has a great potential in therapeutic angiogenesis.

Immobilization and characterization of carbonic anhydrase on the surface of hollow fiber membrane of polymethyl pentene / 生物工程学报

We immobilized carbonic anhydrase (CA) onto the surface of membrane oxygenator of polymethyl pentene (PMP) to enhance the removal of carbon dioxide in blood by two steps. We first introduced hydroxyl groups onto PMP surface by water plasma treatment, and then coupled CA onto PMP surface by using cyanate bromide (CNBr) as a crosslinker. After plasma treatment, the contact angle with water and chemical composition of PMP surface were characterized by analysis system of surface contact angle and XPS. Using p-nitrophenyl acetate (p-NPA) as a substrate, the activity, concentration, storage stability and re-usability of immobilized CA on PMP hollow fibers were studied by ultraviolet spectrophotometer. The preliminary data showed that hydroxyl groups could be introduced on the surface of PMP by water plasma treatment, and CA with catalysis activity could be successfully introduced onto PMP surface in high immobilization efficiency. The activity of covalently immobilized CA increased with the increase of concentration of CNBr, and the maximum was 73% of the theoretical activity of CA spread on PMP surface in monolayer in studied range. Covalently immobilized CA showed higher reusability compared to physically adsorbed CA, and higher storage stability compared to free CA in solution at 37 degrees C. The method would be used potentially in the membrane oxygenator to improve the capacity of removal of carbon dioxide in blood in the future.

Development and application of scoring system for academic conference papers / 中华医学科研管理杂志

Objective To develop a Scoring System for Academic Conference Papers,in order to achieve real-time paper scoring in large academic conference with the information technology and network.Methods Based on Microsoft.Net technology,an information system Was developed,supporting multimeeting.multi-client and dynamic screen show.Results In this system,the conference inforrnation,meeting name and scoring standards Call be customized;the doctor information,papers information,used time,score detail and dynamic list Can be displayed in the screen.This system has been applied in national ophthalmic conferences several times and gained praise from the organizing committee and judges.Conclusions The scoring system for Academic Conference Papers can significantly improve efficiency,help to promote the standardization of paper scoring,and manifest the open,fair and just principles.It is worth of spreading.

Bio-artificial dura mater versus autogenous periosteum for Substituting dura mater / 中国组织工程研究

BACKGROUND:At present,autogenous periosteum and artificial dura mater are usually applied as the substitute grafts for the dural defect by neurological surgery.However they do not accord with the developing trend of modern medicine,due to the limitations of material size and shape,operational complex and additional wound.OBJECTIVE:To observe and compare the evolution of a new type bio-artificial dura and autogenous periosteum in replacing orthotopic duraDESIGN:Controlled observation and trial.SETTING:Animal Testing Center in the 157 Hospital of Guangzhou City.MATERIALS:Nine New Zealand rabbits.aged 6 months and weighed 2-3 kg,either gender was selected.Twelve hybrid healthy dogs of both genders,aged 2 years and weighed 15-20 kg.New type dura mater(No.2006.3460627).METHODS:The experiment was carried out at the Animal Testing Center in the 157 Hospital of Guangzhou City from October 2003 to October 2005.After the general anesthesia and bilateral craniotomy,the bilateral dural defect and pia mater injury were induced partly,then dural neoplasty was performed using new type artificial dura and autogenous periosteum.MAIN OUTCOME MEASURES:At months 1,6,12 of modeling,each three rabbits were selected to isolate and expose the implanted materials,while each four dogs were selected at months 6,12,24 of modeling,died of disease or prior to death.General observation and microscopic assessment of samples were compared to analyze the development of implanted materials at difference stages.RESULTS:Except one experimental dog died during the anesthesia,9 rabbits and 11 dogs were involved in the final presented the extemal surface of adherence and separation with pedcranium,grew well with surrounding orthotopic dura.For the internal surface of materials,the new type artificial dura was more likely the orthotopic dura and did no adhere to pericranium, and filament-shaped adherence appeared occasionally, while there were filament-shaped even month 12 of grafting new type artificial dura into the experimental rabbits.inflammatory cellular reactions such as neutrophil and lymphocyte were not found,additionally no capsule wall formation occurred.The internal surface of artificial dura was covered with epithelial cells,which appeared fibroplasia,fibroblast proliferation,degradation of implants and obvious reduction of total cell amount.Moreover the blood capillary was also found.CONCLUSION:New type artificial dura can achieve the dural reconstruction through producing epithelial cells and being nibbled.degraded and substituted by autogenous tissue.And no adherence to cerebral tissues is found.New type artificial dura is superior to autogenous periosteum for repairing the dural defects.

Application of Typical Case Image Analysis to the Evaluation of Ophthalmologic Clinical Skills / 中华医学教育探索杂志

3-year practice and questionnaire of the graduates majoring in ophthalmology indicate ophthalmologic typical case image implicates very much clinical information,can satisfy ophthalmologic clinical skills test,reflect examinee's clinical knowledge and technical skill level and guide intern to cultivate clinical thinking capability.

Blood compatibility of block copolymer membranes of poly(benzyl L-glutamate)/poly(ethylene glycol) / 生物医学工程学杂志

The blood compatibility of block copolymer membranes of poly(benzyl L-glutamate)/poly(ethylene glycol) and the effect of on the blood compatibility of copolymer were evaluated by the clotting time test, the platelet adhesion and deformation test, and the protein adsorption test. The results showed that in terms of blood compatibility, homopolymer was better than glass and silicone, copolymer was better than homopolymer, and the more the PEG in the copolymer, the better the blood compatibility.

Study on adhesion of platelets to surface of copoly(benzyl glutamate-hydroxy ethyl glutamine) / 生物医学工程学杂志

Copoly (benzyl glutamate-hydroxy ethyl glutamine) were prepared by partially aminolysis of poly benzyl glutamate with hydroxyl ethylamine at 60 degrees C for predeternined period. As aminolysis was proceeding, the content of hydroxy ethyl glutamine in copolymer increased and the yield of aminolysis decreased. When aminolysis time reached 16.5 hours, a copolymer with 0.357 mole fraction of hydroxy ethyl glutamine was obtained. After 16.5 hours a soluble yellow viscous product of reaction was obtained. The aminolysized specimens displayed more swelling degree in water, that is, more hydrophilits. The meassurement of solid-liquid contact angles showed that an increase in critical surface tention with content of hydroxy ethyl glutamine in copolymer was observed. Likewise a obvious increase in polar component and a slight decrease in dispersive of surface free energy, thus an increase in total surface free energy with content of hydroxy ethyl glutamine was found. The platelet adhesion and deformation test indicated that less platelets were adhered to surfaces of all aminolysized specimens than that of both poly benzyl glutamate and polydimethylsilicone. The least adhered platelets on surface of copolymer with 0.133 mole fraction of content of hydroxy ethyl glutamine were observed. The partiall aminolysis of poly benzyl glutamate films is an effective method to improve its hydrophility and antithrombogenicity.

Remote consultation of images of ocular fundus diseases on internet / 中华眼底病杂志

Objective To find convenient methods for remote consultation of images of ocular fundus diseases. Methods A remote consultation system composed of internet was set up.The consultation information,including images,words and figures,was published on internet as web pages,so that the consultants would get the notice and the appointment by e mail.After reading the information on line,the consultants gave their opinions back to internet. Results The remote consultation system of images of ocular fundus diseases was setted up and managed successfully,and 23 patients had been diagnosed by this system. Conclusion The system which has clinical practicality is a simple,quick,effective and economic method for remote consultation of images of ocular fundus diseases. [

Additive Therapeutic Effect of a Combination of Artemether and Daphnetin against Plasmodium berghei in Mice / 中国寄生虫学与寄生虫病杂志

Objective To investigate the therapeutic effect of a combination of artemether and daphnetin against Plasmodium berghei ANKA strain in mice. Methods Groups of P. berghei infected mice were treated with various oral doses of artemether and daphnetin according to “4-day suppress assay”. Thin blood smears were made on the fifth day after inoculation of parasites and the parasitemia reduction rate was calculated. The ED 50 values obtained were plotted on isobolograms. A combined action of artemether and daphnetin was assessed. Results Artemether 0.4mg/(kg?d)?4d exhibited no detectable antimalarial effect, while artemether 0.4mg/(kg?d)?4d combined with daphnetin 7.7 mg/kg.d?4d showed potent antiparasile efficacy. The ED 50s of artemether in combination with daphnetin were lower than that of single artemether or daphnetin. The R-values were higher than 0.4, but lower than 2.7. Conclusion The combination of artemether with daphnetin showed an additive antiparasile effect.

Effect of Daphnetin on SOD Activity and DNA Synthesis of Plasmodium falciparum in vitro / 中国寄生虫学与寄生虫病杂志

Objective To investigate the effect of daphnetin on superoxide dismutase (SOD) activity and DNA synthesis in P. falciparum in vitro. Methods The effect of daphnetin, daphnetin-Fe complex and desferrioxamine B on SOD activity of P. falciparum (P. f) FOCI in vitro was determined with a SOD test-kit. The level of DNA synthesis of P. f synchronized cultured in vitro at various developmental stages after treatment of daphnetin or desferrioxamine B was assayed by fluorescein Hoechest 33258. Results The total SOD activity decreased by 60% after daphnetin treatment while it only decreased by 22% if treated with desferrioxamine B. No effect on SOD activity of P. f treated with daphnetin-Fe complex was observed. The level of DNA synthesis of P. f trophozoites in synchronized in vitro culture was significantly lower than that of the control. Conclusion Daphnetin lowered SOD activity and decreased DNA synthesis of P. f in vitro.